Biomolecular Kinetics: A Step-by-Step Guide (Foundations of Biochemistry and Biophysics) (PDF)

Kinetics underpins the elucidation of biomolecular mechanisms. Advances in instrumentation and sample preparation have opened up the field to a wider audience who often find that the standard textbook treatment is too limited, while books on chemical kinetics are too obscure and detached from the realities of dealing with biological samples. This book brings well-established analytical equations to life by means of numerical simulations that do not require math beyond high-school level. Apart from background theory, the practicalities of making kinetic measurements are discussed, including the widely used stopped-flow method and modern versions of continuous-flow. The advantages and limitations of undertaking single-molecule kinetics are examined. Throughout the text, computational methods are described, including simple spreadsheet programs that are used to illustrate basic concepts and well as a practical means for data and error analysis.